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Thanks! - Andries ________________________________ From: Marek, Laura F [AGRON] Sent: Thursday, February 11, 2016 3:36 PM To: John M. Burke; Andries Temme Cc: Rishi Rajen Masalia; Lisa Donovan Subject: RE: Origin of the SAM 288 (past, present, future) It is true that there are only 191 of the SAM lines in the core 288 or, only 191 core 288 lines in SAM. I believe that all but maybe one of the SAM 288 lines have been sequenced based on emails exchanges in January which included Jennifer, Loren and Nicolas when I asked about the ISA abstract =E2=80=93 which describes the SAM pop as having 271 lines because = that was the number for which there was good data at the time the SAM 2013 paper was written. All of the SAMs have Ames numbers and are not tracked as their originating PI. 1. So everything is equal (since the INRA lines didn=E2= =80=99t have PI sources, they needed some number to be part of the distributions here). 2. Because as John said, there were additional selfings. It was Georgia who said that the SAMs and the PIs are not necessarily equal so I should not be distributing PIs as SAMs. It was far easier (for tracking, for setting up orders) and allowed us to keep the INRAs and the SAMs together, to give everything a new Ames number than to give the PI derived SAMs a different lot code. They do not have new PI numbers (yet) because Peter Bretting said I could not give them PI numbers. Does that answer your questions, Andries? Laura Laura Fredrick Marek, PhD Curator, Oil Seeds Crops North Central Regional Plant Introduction Station (NCRPIS) National Plant Germplasm System, ISU and USDA-ARS mail to: 1305 State Ave Ames, IA 50014 lmarek@iastate.edu 515-294-3519 (NCRPIS) 515-294-1903 (FAX) 515-451-7491 (cell) From: John M. Burke [mailto:jmburke@uga.edu] Sent: Thursday, February 11, 2016 2:19 PM To: Andries Temme ; Marek, Laura F [AGRON] Cc: Rishi Rajen Masalia ; Lisa Donovan Subject: Re: Origin of the SAM 288 (past, present, future) I think, but am not 100% sure, that Laura may have assigned new PI numbers to seed that's derived from the selling that we did hear at UGA since that could result in some divergence. That is, for lines that still exhibited substantial segregation, picking out one individual and self in it could result in a rather different genotype can constitution of the subsequent seeds (relative to the avg values for that original accession). I believe that Jen still referred to these all by their original PI designations in her paper, but they may have be pen renamed when the seed was seen back to Ames. That could account for the apparent discrepancies. I am cc'ing Laura to get her thoughts. You might also make some sense of this by spot heckling the records in GRIN,p. Thanks, John On Thursday, February 11, 2016, Andries Temme > wrote: Hi, I've gone through the PI numbers and line names from the Mandell 2011 and Mandell 2013 papers. If you could have a look at the attached file. - 220 of the current 288 SAM lines overlap in PI number with the 271 PI numbers from the Mandell 2013 paper - The column from Laura's sheet listing whether or not the line was in the Mandell 2011 paper "Core288" seems correct (I could find most of the PI numbers from Laura's sheet or the line name in the table from Mandell 2011. Name format issues makes comparisons difficult) - If the column is indeed correct, as I think it is, only 191 of the current SAM-288 are in the Mandell 2011 Core288 So, is this an issue? Is there sequence/marker/SNiP data for the current SAM-288 as in the table from Laura? Thanks, Andries -- Sent from a mobile device. ------=_NextPart_15544368.653201418207 Content-Type: text/rtf; charset="utf-8" Content-Transfer-Encoding: quoted-printable {\rtf1\ansi\ansicpg1252\fromhtml1 \fbidis \deff0{\fonttbl=0A=0D{\f0\fswiss\= fcharset0 Arial;}=0A=0D{\f1\fmodern Courier New;}=0A=0D{\f2\fnil\fcharset2 = Symbol;}=0A=0D{\f3\fmodern\fcharset0 Courier New;}=0A=0D{\f4\fswiss Calibri= ;}=0A=0D{\f5\fswiss\fcharset0 "Calibri";}}=0A=0D{\colortbl\red0\green0\blue= 0;\red0\green0\blue255;\red0\green0\blue0;}=0A=0D\uc1\pard\plain\deftab360 = \f0\fs24 =0A=0D{\*\htmltag19 }=0A=0D{\*\htmltag2 \par }=0A=0D{\*\html= tag34 }=0A=0D{\*\htmltag1 \par }=0A=0D{\*\htmltag1 \par }=0A=0D{\*\ht= mltag241

Hi Laura,


Great, that makes things clearer!


Thanks!


- Andries



From: Marek, Laura F [AGRON= ] <lmarek@iastate.edu>
Sent: Thursday, February 11, 2016 3:36 PM
To: John M. Burke; Andries Temme
Cc: Rishi Rajen Masalia; Lisa Donovan
Subject: RE: Origin of the SAM 288 (past, present, future)
 

It is true that there are only 191 of the SAM lines in the= core 288 or, only 191 core 288 lines in SAM.  I believe that all but = maybe one of the SAM 288 lines have been sequenced based on emails exchanges in January which included Jennifer, Loren and Ni= colas when I asked about the ISA abstract – which describes the SAM p= op as having 271 lines because that was the number for which there was good= data at the time the SAM 2013 paper was written.

 

All of the SAMs have Ames numbers and are not tracked as t= heir originating PI.  1.  So everything is equal (since the INRA = lines didn’t have PI sources, they needed some number to be part of the distributions here).  2.  Because as John said= , there were additional selfings.  It was Georgia who said that the SA= Ms and the PIs are not necessarily equal so I should not be distributing PI= s as SAMs.  It was far easier (for tracking, for setting up orders) and allowed us to keep the INRAs and the SAMs together,= to give everything a new Ames number than to give the PI derived SAMs a di= fferent lot code.  They do not have new PI numbers (yet) because Peter= Bretting said I could not give them PI numbers.

 

Does that answer your questions, Andries?

 

Laura

Laura Fredrick Marek, PhD

Curator, Oil Seeds Crops

North Central Regional Plant Introduction Station (NCRPIS)=

National Plant Germplasm System, ISU and USDA-ARS

mail to: 1305 State Ave

Ames, IA 50014

lmarek@iastate.edu

 

515-294-3519 (NCRPIS)

515-294-1903 (FAX)

515-451-7491 (cell)

 

From: John M. Burke [mailto:jmburke@uga.edu]
Sent: Thursday, February 11, 2016 2:19 PM
To: Andries Temme <atemme@uga.edu>; Marek, Laura F [AGRON] <= ;lmarek@iastate.edu>
Cc: Rishi Rajen Masalia <masalia@uga.edu>; Lisa Donovan <ld= onovan@uga.edu>
Subject: Re: Origin of the SAM 288 (past, present, future)

 

I think, but am not 100% sure, that Laura may have assigned new PI numbers = to seed that's derived from the selling that we did hear at UGA since that = could result in some divergence. That is, for lines that still exhibited&nb= sp;substantial segregation, picking out one individual and self in it could result in a rather different genotype = can constitution of the subsequent seeds (relative to the avg values for th= at original accession). I believe that Jen still referred to these all by t= heir original PI designations in her paper, but they may have be pen renamed when the seed was seen back to= Ames. That could account for the apparent discrepancies. I am cc'ing Laura= to get her thoughts. You might also make some sense of this by spot heckli= ng the records in GRIN,p. Thanks, John


On Thursday, February 11, 2016, Andries Temme <atemme@uga.edu<= /a>> wrote:

Hi,

 

I've gone through the PI numbers and line names = from the Mandell 2011 and Mandell 2013 papers. If you could have a look at = the attached file.

 

- 220 of the current 288 SAM lines overlap = in PI number with the 271 PI numbers from the Mandell 2013 paper

 

- The column from Laura's sheet listing whether = or not the line was in the Mandell 2011 paper "Core288" seems cor= rect (I could find most of the PI numbers from Laura's sheet or the line name in the table from Mandell 2011. Name format issues makes = comparisons difficult)

 

- If the column is indeed correct, as I thi= nk it is, only  191 of the current SAM-288 are in the Mandel= l 2011 Core288

 

So, is this an issue? Is there sequence/marker/S= NiP data for the current SAM-288 as in the table from Laura?

 

Thanks, Andries



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